Device

Part:BBa_K2483002:Design

Designed by: Bryan Nowack   Group: iGEM17_Potsdam   (2017-09-07)


Lac regulated dCas9 with LacI constitutively expressed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1258
    Illegal BamHI site found at 4945
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were not sure about the strength of the LacI promoter so we just used the Promoter designed in a different plasmid with LacI.


Source

the promoter for LacI was taken from the pRep4 vector, dCas9 from the vector "pdcas9-bacteria". LacI cds was taken form the part BBa_C0012 and the Lac promoter for dCas9 is BBa_R0010.

References